› 4hjf

X-ray diffraction
1.75Å resolution

EAL domain of phosphodiesterase PdeA in complex with c-di-GMP and Ca++

Released:
DOI: 10.2210/pdb4hjf/pdb
PDB entry 4hjf coloured by chain, front view.
PDB entry 4hjf coloured by chain, side view.
PDB entry 4hjf coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Massa C, Schirmer T, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GGDEF family protein Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 36.78 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9A310 (Residues: 240-555; Coverage: 57%)
Gene name: CC_3396
Sequence domains: EAL domain
Structure domains: EAL domain

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand C2E 1 x C2E
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 62.202Å b: 63.186Å c: 66.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.214
Expression system: Escherichia coli BL21(DE3)

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