4hjj

X-ray diffraction
2.1Å resolution

Structure Reveals Function of the Dual Variable Domain Immunoglobulin (DVD-Ig) Molecule

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-212018 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-18 Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 18.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14116 (Residues: 37-192; Coverage: 81%)
Gene names: IGIF, IL18, IL1F4
Sequence domains: Interleukin-1 / 18
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Anti-IL12 Anti-IL18 DFab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 348 amino acids
Theoretical weight: 37.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Anti-IL12 Anti-IL18 DFab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 327 amino acids
Theoretical weight: 35.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 274.385Å b: 65.498Å c: 78.802Å
α: 90° β: 95.2° γ: 90°
R-values:
R R work R free
0.186 0.184 0.22
Expression systems:
  • Escherichia coli
  • Homo sapiens