› 4hjw

X-ray diffraction
2.6Å resolution

Crystal structure of Metarhizium anisopliae IDCase in apo form

Released:
DOI: 10.2210/pdb4hjw/pdb
PDB entry 4hjw coloured by chain, front view.
PDB entry 4hjw coloured by chain, side view.
PDB entry 4hjw coloured by chain, top view.
Source organism: Metarhizium anisopliae
Primary publication:
Crystal structures of isoorotate decarboxylases reveal a novel catalytic mechanism of 5-carboxyl-uracil decarboxylation and shed light on the search for DNA decarboxylase.
Xu S, Li W, Zhu J, Wang R, Li Z, Xu GL, Ding J
Cell Res 23 1296-309 (2013)
PMID: 23917530

Function and Biology Details

Reaction catalysed: 
Uracil 5-carboxylate = uracil + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 378 amino acids
Theoretical weight: 41.35 KDa
Source organism: Metarhizium anisopliae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E9F0X0 (Residues: 1-377; Coverage: 100%)
Gene name: MAA_05919
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3112
Unit cell:
a: 132.984Å b: 132.984Å c: 175.367Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Cancer development, progression, and therapy: an epigenetic overview.
Sarkar et al. (2013)
10 more

3 mentions without citation

Analysis of Human RSV Immunity at the Molecular Level: Learning from the Past and Present.
Vaughan et al. (2015)
2 more