› 4hjx

X-ray diffraction
2.91Å resolution

Crystal structure of F2YRS complexed with F2Y

Released:
DOI: 10.2210/pdb4hjx/pdb
PDB entry 4hjx coloured by chain, front view.
PDB entry 4hjx coloured by chain, side view.
PDB entry 4hjx coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
A genetically encoded 19F NMR probe for tyrosine phosphorylation.
Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J, Xiong Y, Sun J, Gong W, Tian C, Wang J
Angew Chem Int Ed Engl 52 3958-62 (2013)
PMID: 23450644

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 36.05 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 1-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand F2Y 2 x F2Y
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43
Unit cell:
a: 101.307Å b: 101.307Å c: 71.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.268
Expression system: Escherichia coli

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Citations

4 review citations

Advances in recombinant protein expression for use in pharmaceutical research.
Assenberg et al. (2013)
3 more

2 mentions without citation

DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe.
Liu et al. (2020)
1 more