› 4hka

X-ray diffraction
2.7Å resolution

Crystal structure of Drosophila melanogaster tryptophan 2,3-dioxygenase in complex with HEME

Released:
DOI: 10.2210/pdb4hka/pdb
PDB entry 4hka coloured by chain, front view.
PDB entry 4hka coloured by chain, side view.
PDB entry 4hka coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of Drosophila melanogaster tryptophan 2,3-dioxygenase reveals insights into substrate recognition and catalytic mechanism.
Huang W, Gong Z, Li J, Ding J
J Struct Biol 181 291-9 (2013)
PMID: 23333332

Function and Biology Details

Reaction catalysed: 
L-tryptophan + O(2) = N-formyl-L-kynurenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148959 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 2,3-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 43.36 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20351 (Residues: 24-379; Coverage: 94%)
Gene names: CG5163, v
Sequence domains: Tryptophan 2,3-dioxygenase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6422
Unit cell:
a: 114.719Å b: 114.719Å c: 213.438Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.264
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

A short history of heme dioxygenases: rise, fall and rise again.
Raven EL. (2017)
2 more