› 4hl7

X-ray diffraction
1.8Å resolution

Crystal structure of nicotinate phosphoribosyltransferase (target NYSGR-026035) from Vibrio cholerae

Released:
DOI: 10.2210/pdb4hl7/pdb
PDB entry 4hl7 coloured by chain, front view.
PDB entry 4hl7 coloured by chain, side view.
PDB entry 4hl7 coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Mulichak AM, Sauder JM, Keefe LJ, Burley SK, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-191980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 51.9 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KN67 (Residues: 2-435; Coverage: 100%)
Gene names: VC_A0098, pncB
Sequence domains:
Structure domains: nicotinate phosphoribosyltransferase

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21212
Unit cell:
a: 83.42Å b: 147.64Å c: 74.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.201
Expression system: Escherichia coli

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