› 4hmk

X-ray diffraction
3Å resolution

Crystal structure of LeuT-E290S with bound Br

Released:
DOI: 10.2210/pdb4hmk/pdb
PDB entry 4hmk coloured by chain, front view.
PDB entry 4hmk coloured by chain, side view.
PDB entry 4hmk coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
The chloride binding site of Neurotransmitter Sodium Symporters
Kantcheva AK, Quick M, Shi L, Winther AML, Stolzenberg S, Weinstein H, Javitch JA, Nissen P
Proc. Natl. Acad. Sci. U.S.A. (2013)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 57.41 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

4 bound ligands:
Ligand LEU 2 x LEU
Ligand NA 4 x NA
Ligand BR 11 x BR
Ligand BOG 9 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 84.14Å b: 92.32Å c: 87.21Å
α: 90° β: 94.25° γ: 90°
R-values:
R R work R free
0.208 0.205 0.261
Expression system: Escherichia coli

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