› 4hms

X-ray diffraction
1.33Å resolution

Crystal structure of PhzG from Pseudomonas fluorescens 2-79 in complex with a second FMN in the substrate binding site

Released:
DOI: 10.2210/pdb4hms/pdb
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PDB entry 4hms coloured by chain, front view.
PDB entry 4hms coloured by chain, side view.
PDB entry 4hms coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis.
Xu N, Ahuja EG, Janning P, Mavrodi DV, Thomashow LS, Blankenfeldt W
Acta Crystallogr D Biol Crystallogr 69 1403-13 (2013)
PMID: 23897464

Function and Biology Details

Reaction catalysed: 
(1) (1R,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylate + O2 =(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate + H2O2.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrophenazinedicarboxylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.25 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51793 (Residues: 1-222; Coverage: 100%)
Gene name: phzG
Sequence domains:
Structure domains: Electron Transport, Fmn-binding Protein; Chain A

Ligands and Environments


Cofactor: Ligand FMN 3 x FMN
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 57.74Å b: 63.69Å c: 133.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.121 0.154
Expression system: Escherichia coli

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Citations

3 review citations

Phenazines in plant-beneficial Pseudomonas spp.: biosynthesis, regulation, function and genomics.
Biessy et al. (2018)
2 more

3 mentions without citation

Promising Performance of 4HMS, a New Zirconium-89 Octadendate Chelator.
Alnahwi et al. (2020)
2 more

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