› 4hor

X-ray diffraction
1.86Å resolution

Crystal Structure of Full-Length Human IFIT5 with 5`-triphosphate Oligocytidine

Released:
DOI: 10.2210/pdb4hor/pdb
PDB entry 4hor coloured by chain, front view.
PDB entry 4hor coloured by chain, side view.
PDB entry 4hor coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for viral 5'-PPP-RNA recognition by human IFIT proteins.
Abbas YM, Pichlmair A, Górna MW, Superti-Furga G, Nagar B
Nature 494 60-4 (2013)
PMID: 23334420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171639 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Interferon-induced protein with tetratricopeptide repeats 5 Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 55.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13325 (Residues: 1-482; Coverage: 100%)
Gene names: IFIT5, ISG58, RI58
Sequence domains:
Structure domains: Tetratricopeptide repeat domain
RNA (5'-R(*(CTP)P*CP*CP*CP*C)-3') Chain: X
Molecule details ›
Chain: X
Length: 5 nucleotides
Theoretical weight: 1.64 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 54.588Å b: 84.903Å c: 61.47Å
α: 90° β: 106.92° γ: 90°
R-values:
R R work R free
0.167 0.164 0.201
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

40 review citations

mRNA capping: biological functions and applications.
Ramanathan et al. (2016)
39 more

8 mentions without citation

IFIT1: A dual sensor and effector molecule that detects non-2'-O methylated viral RNA and inhibits its translation.
Diamond MS. (2014)
7 more