› 4hpg

X-ray diffraction
2.54Å resolution

Crystal structure of a glycosylated beta-1,3-glucanase (HEV B 2), an allergen from Hevea brasiliensis

Released:
DOI: 10.2210/pdb4hpg/pdb
PDB entry 4hpg coloured by chain, front view.
PDB entry 4hpg coloured by chain, side view.
PDB entry 4hpg coloured by chain, top view.
Source organism: Hevea brasiliensis
Primary publication:
Structural analysis of the endogenous glycoallergen Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis and its recognition by human basophils.
Rodríguez-Romero A, Hernández-Santoyo A, Fuentes-Silva D, Palomares LA, Muñoz-Cruz S, Yépez-Mulia L, Orozco-Martínez S
Acta Crystallogr D Biol Crystallogr 70 329-41 (2014)
PMID: 24531467

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
glucan endo-1,3-beta-D-glucosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 316 amino acids
Theoretical weight: 35.32 KDa
Source organism: Hevea brasiliensis
UniProt:
  • Canonical: A2TM14 (Residues: 37-352; Coverage: 94%)
Sequence domains: Glycosyl hydrolases family 17
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand PEG 2 x PEG
Ligand NAG 1 x NAG
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 87.176Å b: 89.778Å c: 101.542Å
α: 90° β: 113.59° γ: 90°
R-values:
R R work R free
0.195 0.193 0.24

Quick links

Citations

1 review citation

The concept of allergen-associated molecular patterns (AAMP).
Pali-Schöll et al. (2016)
4 other articles

4 mentions without citation

Interfaces between allergen structure and diagnosis: know your epitopes.
Pomés et al. (2015)
3 more