› 4hpv

X-ray diffraction
2.21Å resolution

Crystal structure of S-Adenosylmethionine synthetase from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb4hpv/pdb
PDB entry 4hpv coloured by chain, front view.
PDB entry 4hpv coloured by chain, side view.
PDB entry 4hpv coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Understanding molecular recognition of promiscuity of thermophilic methionine adenosyltransferase sMAT from Sulfolobus solfataricus.
Wang F, Singh S, Zhang J, Huber TD, Helmich KE, Sunkara M, Hurley KA, Goff RD, Bingman CA, Morris AJ, Thorson JS, Phillips GN
FEBS J 281 4224-39 (2014)
PMID: 24649856

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 45.33 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980S9 (Residues: 1-404; Coverage: 100%)
Gene names: SSO0199, mat
Sequence domains: S-adenosylmethionine synthetase (AdoMet synthetase)
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P6522
Unit cell:
a: 151.304Å b: 151.304Å c: 221.145Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.199 0.22
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Methyltransferases: Functions and Applications.
Abdelraheem et al. (2022)
1 more

4 mentions without citation

AdoMet analog synthesis and utilization: current state of the art.
Huber et al. (2016)
3 more