4hqb

X-ray diffraction
2.3Å resolution

Crystal structure of DdrB from Deinococcus radiodurans bound to ssDNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero heptamer (preferred)
hetero tetradecamer
PDBe Complex ID:
PDB-CPX-117604 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Single-stranded DNA-binding protein DdrB Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 148 amino acids
Theoretical weight: 16.92 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RY80 (Residues: 1-144; Coverage: 77%)
Gene names: DR_0070, ddrB
Sequence domains: DdrB-like protein
5'-D(*TP*TP*TP*T)-3' Chain: M
Molecule details ›
Chain: M
Length: 4 nucleotides
Theoretical weight: 1.17 KDa
Source organism: Deinococcus radiodurans
Expression system: Not provided
5'-D(P*TP*TP*TP*TP*T)-3' Chain: N
Molecule details ›
Chain: N
Length: 5 nucleotides
Theoretical weight: 1.48 KDa
Source organism: Deinococcus radiodurans
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P32
Unit cell:
a: 110.7Å b: 110.7Å c: 58.776Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.19 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided