› 4hqe

X-ray diffraction
2.3Å resolution

The crystal structure of QsrR-DNA complex

Released:
DOI: 10.2210/pdb4hqe/pdb
PDB entry 4hqe coloured by chain, front view.
PDB entry 4hqe coloured by chain, side view.
PDB entry 4hqe coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR.
Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, Cho H, Brugarolas P, Gao YN, Peterson SN, Lan L, Bae T, He C
Proc Natl Acad Sci U S A 110 5010-5 (2013)
PMID: 23479646

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101991 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH hxlR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.1 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JWI0 (Residues: 1-112; Coverage: 100%)
Gene name: SAV2123
Sequence domains: HxlR-like helix-turn-helix
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*GP*TP*AP*TP*AP*AP*TP*AP*AP*TP*TP*AP*TP*AP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.22 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*GP*TP*AP*TP*AP*AP*TP*TP*AP*TP*TP*AP*TP*AP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.18 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41
Unit cell:
a: 48.189Å b: 48.189Å c: 156.807Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Thiol-based redox switches in prokaryotes.
Hillion et al. (2015)
4 more

5 mentions without citation

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
4 more