› 4hri

X-ray diffraction
2.95Å resolution

Crystal structure of HetR in complex with a 21-bp palindromic DNA at the upstream of the hetP promoter from Anabaena

Released:
DOI: 10.2210/pdb4hri/pdb
PDB entry 4hri coloured by chain, front view.
PDB entry 4hri coloured by chain, side view.
PDB entry 4hri coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Hu HX, Jiang YL, Zhao MX, Chen Y, Zhang CC, Zhou CZ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117610 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional activator HetR Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 36.03 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: P27709 (Residues: 1-299; Coverage: 100%)
Gene names: alr2339, hetR
Sequence domains:
Structure domains:
DNA (5'-D(P*GP*CP*GP*AP*GP*GP*GP*GP*TP*CP*TP*AP*AP*CP*CP*CP*CP*TP*CP*AP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Not provided
DNA (5'-D(P*AP*TP*GP*AP*GP*GP*GP*GP*TP*TP*AP*GP*AP*CP*CP*CP*CP*TP*CP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 89.883Å b: 89.883Å c: 242.288Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.23 0.307
Expression systems:
  • Escherichia coli
  • Not provided

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