› 4hrm

X-ray diffraction
3.2Å resolution

Structural Basis for Eliciting a Cytotoxic Effect in HER2-Overexpressing Cancer Cells via Binding to the Extracellular Domain of HER2

Released:
DOI: 10.2210/pdb4hrm/pdb
PDB entry 4hrm coloured by chain, front view.
PDB entry 4hrm coloured by chain, side view.
PDB entry 4hrm coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2.
Jost C, Schilling J, Tamaskovic R, Schwill M, Honegger A, Plückthun A
Structure 21 1979-91 (2013)
PMID: 24095059

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138085 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor tyrosine-protein kinase erbB-2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 198 amino acids
Theoretical weight: 22.21 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04626 (Residues: 24-219; Coverage: 16%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains: Receptor L domain
Structure domains: Receptor L-domain
Designed Ankyrin Repeat Protein 9_26 Chains: B, D
Molecule details ›
Chains: B, D
Length: 172 amino acids
Theoretical weight: 18.65 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 138.5Å b: 60.7Å c: 107.2Å
α: 90° β: 118.9° γ: 90°
R-values:
R R work R free
0.315 0.314 0.338
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

8 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
7 more

1 mention without citation

Rigidity of the extracellular part of HER2: Evidence from engineering subdomain interfaces and shared-helix DARPin-DARPin fusions.
Jost et al. (2017)