› 4hsc

X-ray diffraction
2.1Å resolution

Crystal structure of a cholesterol dependent cytolysin

Released:
DOI: 10.2210/pdb4hsc/pdb
PDB entry 4hsc coloured by chain, front view.
PDB entry 4hsc coloured by chain, side view.
PDB entry 4hsc coloured by chain, top view.
Source organism: Streptococcus pyogenes SSI-1
Primary publication:
Structural studies of Streptococcus pyogenes streptolysin O provide insights into the early steps of membrane penetration.
Feil SC, Ascher DB, Kuiper MJ, Tweten RK, Parker MW
J Mol Biol 426 785-92 (2014)
PMID: 24316049

Function and Biology Details

Reaction catalysed: 
NAD(+) + H(2)O = ADP-D-ribose + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptolysin O Chain: X
Molecule details ›
Chain: X
Length: 571 amino acids
Theoretical weight: 63.72 KDa
Source organism: Streptococcus pyogenes SSI-1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DF97 (Residues: 1-571; Coverage: 100%)
Gene names: SPs0132, slo
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 46.208Å b: 85.343Å c: 81.223Å
α: 90° β: 92.08° γ: 90°
R-values:
R R work R free
0.224 0.222 0.264
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
7 more

15 mentions without citation

Cholesterol-dependent cytolysins: from water-soluble state to membrane pore.
Christie et al. (2018)
14 more