› 4hsr

X-ray diffraction
2.13Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164553 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
glutaryl-7-aminocephalosporanic acid acylase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.85 KDa
Source organism: Pseudomonas
Expression system: Escherichia coli BL21(DE3)
Structure domains:
glutaryl-7-aminocephalosporanic acid acylase beta chain Chain: B
Molecule details ›
Chain: B
Length: 543 amino acids
Theoretical weight: 59.33 KDa
Source organism: Pseudomonas
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GLJ 1 x GLJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 68.161Å b: 77.018Å c: 192.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 citation in other articles

Computational design of variants for cephalosporin C acylase from Pseudomonas strain N176 with improved stability and activity.
Tian et al. (2017)
10 more

1 mention without citation

Modelling of substrate access and substrate binding to cephalosporin acylases.
Ferrario et al. (2019)