› 4hst

X-ray diffraction
1.57Å resolution

Crystal structure of a double mutant of a class III engineered cephalosporin acylase

Released:
DOI: 10.2210/pdb4hst/pdb
PDB entry 4hst coloured by chain, front view.
PDB entry 4hst coloured by chain, side view.
PDB entry 4hst coloured by chain, top view.
Source organism: Pseudomonas
Primary publication:
Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity.
Golden E, Paterson R, Tie WJ, Anandan A, Flematti G, Molla G, Rosini E, Pollegioni L, Vrielink A
Biochem J 451 217-26 (2013)
PMID: 23373797

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164554 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
glutaryl-7-aminocephalosporanic acid acylase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.85 KDa
Source organism: Pseudomonas
Expression system: Escherichia coli BL21(DE3)
Structure domains:
glutaryl-7-aminocephalosporanic acid acylase beta chain Chain: B
Molecule details ›
Chain: B
Length: 543 amino acids
Theoretical weight: 59.23 KDa
Source organism: Pseudomonas
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GLJ 1 x GLJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 68.385Å b: 77.845Å c: 191.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.121 0.119 0.162
Expression system: Escherichia coli BL21(DE3)

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Citations

11 citation in other articles

Computational design of variants for cephalosporin C acylase from Pseudomonas strain N176 with improved stability and activity.
Tian et al. (2017)
10 more

2 mentions without citation

A global map of the protein shape universe.
Han et al. (2019)
1 more