› 4ht4

X-ray diffraction
2.91Å resolution

Molecular Basis of Vancomycin Resistance Transfer in Staphylococcus aureus

Released:
DOI: 10.2210/pdb4ht4/pdb
PDB entry 4ht4 coloured by chain, front view.
PDB entry 4ht4 coloured by chain, side view.
PDB entry 4ht4 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Molecular basis of antibiotic multiresistance transfer in Staphylococcus aureus.
Edwards JS, Betts L, Frazier ML, Pollet RM, Kwong SM, Walton WG, Ballentine WK, Huang JJ, Habibi S, Del Campo M, Meier JL, Dervan PB, Firth N, Redinbo MR
Proc Natl Acad Sci U S A 110 2804-9 (2013)
PMID: 23359708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117612 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
MobA/MobL protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 23.07 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q53632 (Residues: 2-195; Coverage: 29%)
Gene name: nes
Sequence domains: MobA/MobL family
Structure domains: BirA Bifunctional Protein; domain 2
DNA (28-MER) Chain: B
Molecule details ›
Chain: B
Length: 28 nucleotides
Theoretical weight: 8.64 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NI 1 x NI
Ligand CA 8 x CA
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P6422
Unit cell:
a: 63.226Å b: 63.226Å c: 313.756Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.286
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

5 review citations

Staphylococcus aureus genomics and the impact of horizontal gene transfer.
Lindsay JA. (2014)
4 more

11 mentions without citation

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.
Chandler et al. (2013)
10 more