› 4ht8

X-ray diffraction
1.9Å resolution

Crystal structure of E coli Hfq bound to poly(A) A7

Released:
DOI: 10.2210/pdb4ht8/pdb
PDB entry 4ht8 coloured by chain, front view.
PDB entry 4ht8 coloured by chain, side view.
PDB entry 4ht8 coloured by chain, top view.
Source organisms:
Primary publication:
Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA.
Wang W, Wang L, Wu J, Gong Q, Shi Y
Nucleic Acids Res 41 5938-48 (2013)
PMID: 23605038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-164555 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein hfq Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 65 amino acids
Theoretical weight: 7.33 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
Structure domains: SH3 type barrels.
RNA (5'-R(*AP*AP*AP*AP*AP*AP*A)-3') Chains: I, K
Molecule details ›
Chains: I, K
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 103.99Å b: 40.71Å c: 100.85Å
α: 90° β: 101.36° γ: 90°
R-values:
R R work R free
0.269 0.19 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Hfq: the flexible RNA matchmaker.
Updegrove et al. (2016)
2 more

5 mentions without citation

Structural model of an mRNA in complex with the bacterial chaperone Hfq.
Peng et al. (2014)
4 more