› 4ht9

X-ray diffraction
1.8Å resolution

Crystal structure of E coli Hfq bound to two RNAs

Released:
DOI: 10.2210/pdb4ht9/pdb
Model geometry
Fit model/data
PDB entry 4ht9 coloured by chain, front view.
PDB entry 4ht9 coloured by chain, side view.
PDB entry 4ht9 coloured by chain, top view.
Source organisms:
Primary publication:
Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA.
Wang W, Wang L, Wu J, Gong Q, Shi Y
OpenAccess logo Nucleic Acids Res 41 5938-48 (2013)
PMID: 23605038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-164556 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Protein hfq Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 65 amino acids
Theoretical weight: 7.33 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli
Structure domains: SH3 type barrels.
RNA (5'-R(*AP*AP*AP*AP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(*AP*UP*UP*UP*UP*UP*UP*A)-3') Chain: E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Unit cell:
a: 60.59Å b: 38.28Å c: 84.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Hfq: the flexible RNA matchmaker.
Updegrove et al. (2016)
2 more

4 mentions without citation

Recognition of the small regulatory RNA RydC by the bacterial Hfq protein.
Dimastrogiovanni et al. (2014)
3 more