› 4hte

X-ray diffraction
3Å resolution

Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus

Released:
DOI: 10.2210/pdb4hte/pdb
PDB entry 4hte coloured by chain, front view.
PDB entry 4hte coloured by chain, side view.
PDB entry 4hte coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Molecular basis of antibiotic multiresistance transfer in Staphylococcus aureus.
Edwards JS, Betts L, Frazier ML, Pollet RM, Kwong SM, Walton WG, Ballentine WK, Huang JJ, Habibi S, Del Campo M, Meier JL, Dervan PB, Firth N, Redinbo MR
Proc Natl Acad Sci U S A 110 2804-9 (2013)
PMID: 23359708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MobA/MobL protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 42.17 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O87361 (Residues: 254-593; Coverage: 51%)
Gene names: BN1321_460007, GQX37_05950, PGO1_p08, SAMEA2078260_02856, SAMEA2081063_02873, SAP014A_018, SAP015D_002, SAP068A_007, SAP069A_033, SAP079A_020, SAP080A_038, SAP082A_025, VRA0057, nes
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 75.284Å b: 75.284Å c: 179.849Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.267 0.292
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.
Chandler et al. (2013)
6 more

2 mentions without citation

Molecular basis of antibiotic multiresistance transfer in Staphylococcus aureus.
Edwards et al. (2013)
1 more