› 4htg

X-ray diffraction
1.45Å resolution

Porphobilinogen Deaminase from Arabidopsis Thaliana

Released:
DOI: 10.2210/pdb4htg/pdb
PDB entry 4htg coloured by chain, front view.
PDB entry 4htg coloured by chain, side view.
PDB entry 4htg coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Insights into the mechanism of pyrrole polymerization catalysed by porphobilinogen deaminase: high-resolution X-ray studies of the Arabidopsis thaliana enzyme.
Roberts A, Gill R, Hussey RJ, Mikolajek H, Erskine PT, Cooper JB, Wood SP, Chrystal EJ, Shoolingin-Jordan PM
Acta Crystallogr D Biol Crystallogr 69 471-85 (2013)
PMID: 23519422

Function and Biology Details

Reaction catalysed: 
4 porphobilinogen + H(2)O = hydroxymethylbilane + 4 NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-174990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porphobilinogen deaminase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 34.66 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q43316 (Residues: 63-382; Coverage: 84%)
Gene names: At5g08280, F8L15_10, HEMC, RUG1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand 18W 1 x 18W
1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 141.573Å b: 37.271Å c: 55.069Å
α: 90° β: 105° γ: 90°
R-values:
R R work R free
0.149 0.145 0.217
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
4 other articles

10 mentions without citation

Acute Intermittent Porphyria: An Overview of Therapy Developments and Future Perspectives Focusing on Stabilisation of HMBS and Proteostasis Regulators.
Bustad et al. (2021)
9 more