› 4htl

X-ray diffraction
1.64Å resolution

Lmo2764 protein, a putative N-acetylmannosamine kinase, from Listeria monocytogenes

Released:
DOI: 10.2210/pdb4htl/pdb
PDB entry 4htl coloured by chain, front view.
PDB entry 4htl coloured by chain, side view.
PDB entry 4htl coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Entry authors: Osipiuk J, Mack J, Endres M, Salazar J, Zhang W, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + cellobiose = ADP + 6-phospho-beta-D-glucosyl-(1->4)-D-glucose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-186931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucoside kinase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.82 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Y3R9 (Residues: 1-294; Coverage: 100%)
Gene names: bglK, lmo2764
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: F222
Unit cell:
a: 87.694Å b: 121.989Å c: 153.297Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.128 0.169
Expression system: Escherichia coli BL21(DE3)

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