› 4hu0

X-ray diffraction
2.38Å resolution

Crystal Structure of a metagenome-derived cellulase Cel5A in complex with cellotetraose

Released:
DOI: 10.2210/pdb4hu0/pdb
PDB entry 4hu0 coloured by chain, front view.
PDB entry 4hu0 coloured by chain, side view.
PDB entry 4hu0 coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Zhuang N, Lee KH

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 359 amino acids
Theoretical weight: 41.68 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6PLH5 (Residues: 26-363; Coverage: 100%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand GOL 6 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P213
Unit cell:
a: 136.924Å b: 136.924Å c: 136.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.188
Expression system: Escherichia coli BL21(DE3)

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