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› 4hu5

X-ray diffraction
2.3Å resolution

Oxime side-chain cross-links in the GCN4-p1 dimeric coiled coil: Linear precursor

Released:
DOI: 10.2210/pdb4hu5/pdb
Model geometry
Fit model/data
PDB entry 4hu5 coloured by chain, front view.
PDB entry 4hu5 coloured by chain, side view.
PDB entry 4hu5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Oxime side-chain cross-links in an α-helical coiled-coil protein: structure, thermodynamics, and folding-templated synthesis of bicyclic species.
Haney CM, Horne WS
Chemistry 19 11342-51 (2013)
PMID: 23843311

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 35 amino acids
Theoretical weight: 4.08 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P03069 (Residues: 249-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand ACT 3 x ACT
1 modified residue:
Ligand NLE 2 x NLE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 76.729Å b: 30.907Å c: 33.306Å
α: 90° β: 97.75° γ: 90°
R-values:
R R work R free
0.206 0.202 0.268
Expression system: Not provided

Quick links

Citations

3 review citations

Oximes and Hydrazones in Bioconjugation: Mechanism and Catalysis.
Kölmel et al. (2017)
2 more