› 4hu8

X-ray diffraction
2Å resolution

Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut

Released:
DOI: 10.2210/pdb4hu8/pdb
PDB entry 4hu8 coloured by chain, front view.
PDB entry 4hu8 coloured by chain, side view.
PDB entry 4hu8 coloured by chain, top view.
Source organism: Globitermes brachycerastes
Primary publication:
Biochemical characterization and crystal structure of a GH10 xylanase from termite gut bacteria reveal a novel structural feature and significance of its bacterial Ig-like domain.
Han Q, Liu N, Robinson H, Cao L, Qian C, Wang Q, Xie L, Ding H, Wang Q, Huang Y, Li J, Zhou Z
Biotechnol Bioeng 110 3093-103 (2013)
PMID: 23794438

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125616 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylanase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 456 amino acids
Theoretical weight: 51.21 KDa
Source organism: Globitermes brachycerastes
Expression system: Escherichia coli
UniProt:
  • Canonical: K7PDC1 (Residues: 21-476; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 55.685Å b: 86.963Å c: 246.433Å
α: 92.96° β: 90.86° γ: 108.56°
R-values:
R R work R free
0.221 0.219 0.266
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Termites as targets and models for biotechnology.
Scharf ME. (2015)
2 more

2 mentions without citation

Large-Scale Discovery of Microbial Fibrillar Adhesins and Identification of Novel Members of Adhesive Domain Families.
Monzon et al. (2022)
1 more