4huv

X-ray diffraction
2.5Å resolution

Crystal Structure of H2Db-NPM6W

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135018 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 281 amino acids
Theoretical weight: 32.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01899 (Residues: 25-304; Coverage: 83%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Nucleoprotein Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.08 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: Q5Q157 (Residues: 367-375; Coverage: 2%)
Gene name: NP

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 86.28Å b: 153.62Å c: 80.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided