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› 4hw9

X-ray diffraction
4.14Å resolution

Crystal Structure of Helicobacter pylori MscS (Closed State)

Released:
DOI: 10.2210/pdb4hw9/pdb
Model geometry
Fit model/data
PDB entry 4hw9 coloured by chain, front view.
PDB entry 4hw9 coloured by chain, side view.
PDB entry 4hw9 coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 Å and 4.1 Å resolutions.
Lai JY, Poon YS, Kaiser JT, Rees DC
Protein Sci 22 502-9 (2013)
PMID: 23339071

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mechanosensitive channel MscS Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 309 amino acids
Theoretical weight: 34.4 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E8QGV2 (Residues: 1-274; Coverage: 100%)
Gene name: HPIN_05000
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 113.92Å b: 143.1Å c: 178.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.244 0.304
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

United in diversity: mechanosensitive ion channels in plants.
Hamilton et al. (2015)
10 more

10 mentions without citation

Membrane-spanning α-helical barrels as tractable protein-design targets.
Niitsu et al. (2017)
9 more