› 4hxt

X-ray diffraction
1.95Å resolution

Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR329

Released:
DOI: 10.2210/pdb4hxt/pdb
PDB entry 4hxt coloured by chain, front view.
PDB entry 4hxt coloured by chain, side view.
PDB entry 4hxt coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Computational design of self-assembling cyclic protein homo-oligomers.
Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D
Nat Chem 9 353-360 (2017)
PMID: 28338692

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
De Novo Protein OR329 Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 26.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 95.493Å b: 31.941Å c: 72.543Å
α: 90° β: 90.78° γ: 90°
R-values:
R R work R free
0.166 0.164 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
9 more

2 mentions without citation

A general computational approach for repeat protein design.
Parmeggiani et al. (2015)
1 more