› 4hxy

X-ray diffraction
1.68Å resolution

PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803

Released:
DOI: 10.2210/pdb4hxy/pdb
PDB entry 4hxy coloured by chain, front view.
PDB entry 4hxy coloured by chain, side view.
PDB entry 4hxy coloured by chain, top view.
Source organism: Streptomyces sp. HK803
Primary publication:
Structural and stereochemical analysis of a modular polyketide synthase ketoreductase domain required for the generation of a cis-alkene.
Bonnett SA, Whicher JR, Papireddy K, Florova G, Smith JL, Reynolds KA
Chem Biol 20 772-83 (2013)
PMID: 23790488

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 45.54 KDa
Source organism: Streptomyces sp. HK803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6V1M8 (Residues: 1813-2248; Coverage: 18%)
Gene name: plm1
Sequence domains: KR domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NDP 2 x NDP
Ligand ACA 1 x ACA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 42.157Å b: 123.398Å c: 79.884Å
α: 90° β: 90.27° γ: 90°
R-values:
R R work R free
0.156 0.155 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

The structural biology of biosynthetic megaenzymes.
Weissman KJ. (2015)
12 more

3 mentions without citation

Stereocontrol within polyketide assembly lines.
Keatinge-Clay AT. (2016)
2 more