› 4hyo

X-ray diffraction
1.65Å resolution

Crystal Structure of MthK Pore

Released:
DOI: 10.2210/pdb4hyo/pdb
PDB entry 4hyo coloured by chain, front view.
PDB entry 4hyo coloured by chain, side view.
PDB entry 4hyo coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Primary publication:
The voltage-dependent gate in MthK potassium channels is located at the selectivity filter.
Posson DJ, McCoy JG, Nimigean CM
Nat Struct Mol Biol 20 159-66 (2013)
PMID: 23262489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 91 amino acids
Theoretical weight: 10.15 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O27564 (Residues: 11-101; Coverage: 27%)
Gene names: MTH_1520, mthK
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand K 8 x K
Ligand HEZ 12 x HEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 44.035Å b: 63.452Å c: 63.477Å
α: 90.03° β: 89.99° γ: 89.99°
R-values:
R R work R free
0.165 0.164 0.181
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Molecular Determinants of BK Channel Functional Diversity and Functioning.
Latorre et al. (2017)
5 more

9 mentions without citation

Pore size matters for potassium channel conductance.
Naranjo et al. (2016)
8 more