› 4hz1

X-ray diffraction
2.2Å resolution

Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site.

Released:
DOI: 10.2210/pdb4hz1/pdb
PDB entry 4hz1 coloured by chain, front view.
PDB entry 4hz1 coloured by chain, side view.
PDB entry 4hz1 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Azurin as a protein scaffold for a low-coordinate nonheme iron site with a small-molecule binding pocket.
McLaughlin MP, Retegan M, Bill E, Payne TM, Shafaat HS, Peña S, Sudhamsu J, Ensign AA, Crane BR, Neese F, Holland PL
J Am Chem Soc 134 19746-57 (2012)
PMID: 23167247

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.96 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand ACT 5 x ACT
Ligand FE2 2 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 47.309Å b: 98.033Å c: 108.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.277
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Metal-binding promiscuity in artificial metalloenzyme design.
Pordea A. (2015)
2 more