› 4hzg

X-ray diffraction
1.95Å resolution

Structure of haloalkane dehalogenase DhaA from Rhodococcus rhodochrous

Released:
DOI: 10.2210/pdb4hzg/pdb
PDB entry 4hzg coloured by chain, front view.
PDB entry 4hzg coloured by chain, side view.
PDB entry 4hzg coloured by chain, top view.
Source organism: Rhodococcus sp.
Primary publication:
Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31.
Lahoda M, Mesters JR, Stsiapanava A, Chaloupkova R, Kuty M, Damborsky J, Kuta Smatanova I
Acta Crystallogr D Biol Crystallogr 70 209-17 (2014)
PMID: 24531456

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.11 KDa
Source organism: Rhodococcus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3G3 (Residues: 1-293; Coverage: 100%)
Gene name: dhaA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P1
Unit cell:
a: 42.77Å b: 44.47Å c: 46.51Å
α: 115.27° β: 97.77° γ: 109.23°
R-values:
R R work R free
0.219 0.216 0.273
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Properties and biotechnological applications of natural and engineered haloalkane dehalogenases.
Nagata et al. (2015)
2 other articles

2 mentions without citation

Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst.
Markova et al. (2020)
1 more