› 4hzi

X-ray diffraction
1.85Å resolution

Crystal structure of the Leptospira interrogans ATPase subunit of an orphan ABC transporter

Released:
DOI: 10.2210/pdb4hzi/pdb
PDB entry 4hzi coloured by chain, front view.
PDB entry 4hzi coloured by chain, side view.
PDB entry 4hzi coloured by chain, top view.
Source organism: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Primary publication:
Structural and functional characterization of an orphan ATP-binding cassette ATPase involved in manganese utilization and tolerance in Leptospira spp.
Benaroudj N, Saul F, Bellalou J, Miras I, Weber P, Bondet V, Murray GL, Adler B, Ristow P, Louvel H, Haouz A, Picardeau M
J Bacteriol 195 5583-91 (2013)
PMID: 24123817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 34.04 KDa
Source organism: Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72QN4 (Residues: 2-266; Coverage: 100%)
Gene name: LIC_12079
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 58.256Å b: 92.512Å c: 130.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Micronutrients and Leptospirosis: A Review of the Current Evidence.
Herman et al. (2016)
1 other article

2 mentions without citation

Structural and functional characterization of an orphan ATP-binding cassette ATPase involved in manganese utilization and tolerance in Leptospira spp.
Benaroudj et al. (2013)
1 more