› 4hzo

X-ray diffraction
1.76Å resolution

The Structure of the Bifunctional Acetyltransferase/Decarboxylase LnmK from the Leinamycin Biosynthetic Pathway Revealing Novel Activity for a Double Hot Dog Fold

Released:
DOI: 10.2210/pdb4hzo/pdb
PDB entry 4hzo coloured by chain, front view.
PDB entry 4hzo coloured by chain, side view.
PDB entry 4hzo coloured by chain, top view.
Source organism: Streptomyces atroolivaceus
Primary publication:
Structure of the bifunctional acyltransferase/decarboxylase LnmK from the leinamycin biosynthetic pathway revealing novel activity for a double-hot-dog fold.
Lohman JR, Bingman CA, Phillips GN, Shen B
Biochemistry 52 902-11 (2013)
PMID: 23320975

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LnmK N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 35.28 KDa
Source organism: Streptomyces atroolivaceus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGP1 (Residues: 2-319; Coverage: 100%)
Gene name: LnmK
Sequence domains: LnmK N-terminal Hot Dog Fold domain
Structure domains: Hotdog Thioesterase

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P6122
Unit cell:
a: 60.487Å b: 60.487Å c: 310.964Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.22 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biosynthesis of polyketides by trans-AT polyketide synthases.
Helfrich et al. (2016)
14 other articles

3 mentions without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)
2 more