4i0a

X-ray diffraction
2.2Å resolution

structure of the mutant Catabolite gene activator protein V132A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-142283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional dual regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 45.9Å b: 93.3Å c: 104.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.233
Expression system: Escherichia coli