› 4i0k

X-ray diffraction
2.97Å resolution

Crystal structure of murine B7-H3 extracellular domain

Released:
DOI: 10.2210/pdb4i0k/pdb
PDB entry 4i0k coloured by chain, front view.
PDB entry 4i0k coloured by chain, side view.
PDB entry 4i0k coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and T cell inhibition properties of B7 family member, B7-H3.
Vigdorovich V, Ramagopal UA, Lázár-Molnár E, Sylvestre E, Lee JS, Hofmeyer KA, Zang X, Nathenson SG, Almo SC
Structure 21 707-17 (2013)
PMID: 23583036

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
CD276 antigen Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 24.26 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q8VE98 (Residues: 34-247; Coverage: 74%)
Gene names: B7h3, Cd276
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, null
Spacegroup: P6122
Unit cell:
a: 100.92Å b: 100.92Å c: 188.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.25
Expression system: Drosophila melanogaster

Quick links

Citations

22 review citations

B7-H3 role in the immune landscape of cancer.
Castellanos et al. (2017)
21 more

6 mentions without citation

The third group of the B7-CD28 immune checkpoint family: HHLA2, TMIGD2, B7x, and B7-H3.
Janakiram et al. (2017)
5 more