› 4i0u

X-ray diffraction
2.7Å resolution

Improved structure of Thermotoga maritima CorA at 2.7 A resolution

Released:
DOI: 10.2210/pdb4i0u/pdb
PDB entry 4i0u coloured by chain, front view.
PDB entry 4i0u coloured by chain, side view.
PDB entry 4i0u coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Exploring the structure and function of Thermotoga maritima CorA reveals the mechanism of gating and ion selectivity in Co2+/Mg2+ transport.
Nordin N, Guskov A, Phua T, Sahaf N, Xia Y, Lu S, Eshaghi H, Eshaghi S
Biochem J 451 365-74 (2013)
PMID: 23425532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalt/magnesium transport protein CorA Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 351 amino acids
Theoretical weight: 41.5 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZ31 (Residues: 1-351; Coverage: 100%)
Gene names: TM_0561, corA
Sequence domains: CorA-like Mg2+ transporter protein
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 24 x MG
Ligand CL 13 x CL
Ligand LMT 3 x LMT
Ligand PEG 21 x PEG
Ligand PG0 1 x PG0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 116.25Å b: 151.5Å c: 143.36Å
α: 90° β: 98.88° γ: 90°
R-values:
R R work R free
0.231 0.228 0.289
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Hydrophobic gating in ion channels.
Aryal et al. (2015)
2 more

10 mentions without citation

Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating.
Matthies et al. (2016)
9 more