› 4i19

X-ray diffraction
2.15Å resolution

The crystal structure of an epoxide hydrolase from Streptomyces carzinostaticus subsp. neocarzinostaticus.

Released:
DOI: 10.2210/pdb4i19/pdb
PDB entry 4i19 coloured by chain, front view.
PDB entry 4i19 coloured by chain, side view.
PDB entry 4i19 coloured by chain, top view.
Source organism: Streptomyces carzinostaticus subsp. neocarzinostaticus
Entry authors: Tan K, Bigelow L, Clancy S, Babnigg G, Bingman CA, Yennamalli R, Lohman J, Ma M, Shen B, Phillips Jr GN, Joachimiak A, Midwest Center for Structural Genomics (MCSG), Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epoxide hydrolase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.74 KDa
Source organism: Streptomyces carzinostaticus subsp. neocarzinostaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84HB8 (Residues: 1-385; Coverage: 100%)
Sequence domains: Epoxide hydrolase N terminus
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand FMT 10 x FMT
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 76.011Å b: 76.011Å c: 161.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.209
Expression system: Escherichia coli BL21(DE3)

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