› 4i1i

X-ray diffraction
1.5Å resolution

Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin in complex with NAD

Released:
DOI: 10.2210/pdb4i1i/pdb
PDB entry 4i1i coloured by chain, front view.
PDB entry 4i1i coloured by chain, side view.
PDB entry 4i1i coloured by chain, top view.
Source organism: Leishmania major strain Friedlin
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 36.48 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4Q7X6 (Residues: 1-324; Coverage: 100%)
Gene names: LMJF_28_2860, cMDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand PO4 3 x PO4
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 64.82Å b: 65.13Å c: 77.49Å
α: 90° β: 110.67° γ: 90°
R-values:
R R work R free
0.146 0.145 0.166
Expression system: Escherichia coli BL21(DE3)

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