› 4i3g

X-ray diffraction
1.4Å resolution

Crystal Structure of DesR, a beta-glucosidase from Streptomyces venezuelae in complex with D-glucose.

Released:
DOI: 10.2210/pdb4i3g/pdb
PDB entry 4i3g coloured by chain, front view.
PDB entry 4i3g coloured by chain, side view.
PDB entry 4i3g coloured by chain, top view.
Source organism: Streptomyces venezuelae
Primary publication:
The structure of DesR from Streptomyces venezuelae, a β-glucosidase involved in macrolide activation.
Zmudka MW, Thoden JB, Holden HM
Protein Sci 22 883-92 (2013)
PMID: 23225731

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 829 amino acids
Theoretical weight: 87.05 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5IXN7 (Residues: 2-828; Coverage: 100%)
Gene name: desR
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand BGC 2 x BGC
Ligand SO4 12 x SO4
Ligand MPO 2 x MPO
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 131.269Å b: 198.314Å c: 67.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Chitin and chitinase: Role in pathogenicity, allergenicity and health.
Patel et al. (2017)
1 more

8 mentions without citation

Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes.
Guo et al. (2015)
7 more