› 4i3m

X-ray diffraction
1.95Å resolution

Aer2 poly-HAMP domains: L44H HAMP1 CW-lock mutant

Released:
DOI: 10.2210/pdb4i3m/pdb
PDB entry 4i3m coloured by chain, front view.
PDB entry 4i3m coloured by chain, side view.
PDB entry 4i3m coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
HAMP domain conformers that propagate opposite signals in bacterial chemoreceptors.
Airola MV, Sukomon N, Samanta D, Borbat PP, Freed JH, Watts KJ, Crane BR
PLoS Biol 11 e1001479 (2013)
PMID: 23424282

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein McpB Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.01 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I6V6 (Residues: 1-172; Coverage: 25%)
Gene names: PA0176, aer-2, aer2, mcpB, tlpG
Sequence domains: HAMP N-terminal domain 3
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand NA 1 x NA
Ligand CL 1 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3212
Unit cell:
a: 61.058Å b: 61.058Å c: 81.406Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.259
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Signaling and sensory adaptation in Escherichia coli chemoreceptors: 2015 update.
Parkinson et al. (2015)
7 more

7 mentions without citation

HAMP domain conformers that propagate opposite signals in bacterial chemoreceptors.
Airola et al. (2013)
6 more