› 4i4j

X-ray diffraction
2.78Å resolution

The structure of SgcE10, the ACP-polyene thioesterase involved in C-1027 biosynthesis

Released:
DOI: 10.2210/pdb4i4j/pdb
PDB entry 4i4j coloured by chain, front view.
PDB entry 4i4j coloured by chain, side view.
PDB entry 4i4j coloured by chain, top view.
Source organism: Streptomyces globisporus
Primary publication:
Crystal Structure of Thioesterase SgcE10 Supporting Common Polyene Intermediates in 9- and 10-Membered Enediyne Core Biosynthesis.
Annaval T, Rudolf JD, Chang CY, Lohman JR, Kim Y, Bigelow L, Jedrzejczak R, Babnigg G, Joachimiak A, Phillips GN, Shen B
ACS Omega 2 5159-5169 (2017)
PMID: 28884166

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein-like protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 159 amino acids
Theoretical weight: 18.15 KDa
Source organism: Streptomyces globisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GME8 (Residues: 1-156; Coverage: 100%)
Sequence domains: Thioesterase-like superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments

3 bound ligands:
Ligand TAR 3 x TAR
Ligand EDO 1 x EDO
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 57.416Å b: 184.526Å c: 59.401Å
α: 90° β: 95.6° γ: 90°
R-values:
R R work R free
0.215 0.211 0.27
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Acyltransferases as Tools for Polyketide Synthase Engineering.
Musiol-Kroll et al. (2018)
4 other articles

2 mentions without citation

Thioesterase enzyme families: Functions, structures, and mechanisms.
Caswell et al. (2022)
1 more