› 4i4k

X-ray diffraction
1.7Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF4440 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 15.02 KDa
Source organism: Streptomyces globisporus C-1027
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GMG4 (Residues: 1-140; Coverage: 100%)
Sequence domains: Domain of unknown function (DUF4440)
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

5 bound ligands:
Ligand CIT 2 x CIT
Ligand PG4 1 x PG4
Ligand GOL 1 x GOL
Ligand PO4 1 x PO4
Ligand 1PE 1 x 1PE
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 72.715Å b: 86.901Å c: 55.265Å
α: 90° β: 121.58° γ: 90°
R-values:
R R work R free
0.169 0.168 0.195
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis.
Vuksanovic et al. (2020)
5 more

1 mention without citation

Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027.
Huang et al. (2016)