› 4i69

X-ray diffraction
1.79Å resolution

Crystal structure of the K463A mutant of the RRM domain of RNA helicase HERA from T. thermophilus

Released:
DOI: 10.2210/pdb4i69/pdb
PDB entry 4i69 coloured by chain, front view.
PDB entry 4i69 coloured by chain, side view.
PDB entry 4i69 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera.
Steimer L, Wurm JP, Linden MH, Rudolph MG, Wöhnert J, Klostermeier D
Nucleic Acids Res 41 6259-72 (2013)
PMID: 23625962

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat resistant RNA dependent ATPase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 87 amino acids
Theoretical weight: 9.52 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72GF3 (Residues: 431-517; Coverage: 17%)
Gene name: TT_C1895
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P31
Unit cell:
a: 49.221Å b: 49.221Å c: 78.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.195 0.245
Expression system: Escherichia coli

Quick links

Citations

3 review citations

RNA helicase proteins as chaperones and remodelers.
Jarmoskaite et al. (2014)
2 more

1 mention without citation

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera.
Steimer et al. (2013)