› 4i6m

X-ray diffraction
2.8Å resolution

Structure of Arp7-Arp9-Snf2(HSA)-RTT102 subcomplex of SWI/SNF chromatin remodeler.

Released:
DOI: 10.2210/pdb4i6m/pdb
PDB entry 4i6m coloured by chain, front view.
PDB entry 4i6m coloured by chain, side view.
PDB entry 4i6m coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of an actin-related subcomplex of the SWI/SNF chromatin remodeler.
Schubert HL, Wittmeyer J, Kasten MM, Hinata K, Rawling DC, Héroux A, Cairns BR, Hill CP
Proc Natl Acad Sci U S A 110 3345-50 (2013)
PMID: 23401505

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-149519 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Actin-related protein 7 Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 54.61 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12406 (Residues: 1-477; Coverage: 100%)
Gene names: ARP7, SWP61, YP9367.14, YPR034W
Sequence domains: Actin
Structure domains:
Actin-like protein ARP9 Chain: B
Molecule details ›
Chain: B
Length: 439 amino acids
Theoretical weight: 50.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05123 (Residues: 1-467; Coverage: 94%)
Gene names: ARP9, SWP59, YM9973.07, YMR033W
Sequence domains: Actin
Structure domains: Nucleotidyltransferase; domain 5
Transcription regulatory protein SNF2 Chain: C
Molecule details ›
Chain: C
Length: 106 amino acids
Theoretical weight: 12.82 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22082 (Residues: 575-667; Coverage: 6%)
Gene names: GAM1, RIC1, SNF2, SWI2, TYE3, YOR290C
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Regulator of Ty1 transposition protein 102 Chain: D
Molecule details ›
Chain: D
Length: 157 amino acids
Theoretical weight: 18.01 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53330 (Residues: 1-157; Coverage: 100%)
Gene names: G9378, RTT102, YGR275W
Sequence domains: Rtt102p-like transcription regulator protein
Structure domains: Factor Xa Inhibitor

Ligands and Environments

1 bound ligand:
Ligand PO4 12 x PO4
1 modified residue:
Ligand MSE 27 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 227.374Å b: 104.138Å c: 81.319Å
α: 90° β: 93.78° γ: 90°
R-values:
R R work R free
0.186 0.184 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Regulating the chromatin landscape: structural and mechanistic perspectives.
Bartholomew B. (2014)
13 more

12 mentions without citation

Molecular architecture of the ATP-dependent chromatin-remodeling complex SWR1.
Nguyen et al. (2013)
11 more