› 4i7h

X-ray diffraction
2Å resolution

Structural basis for peroxide sensing and gene regulation by PerR from Streptococcus pyogenes

Released:
DOI: 10.2210/pdb4i7h/pdb
PDB entry 4i7h coloured by chain, front view.
PDB entry 4i7h coloured by chain, side view.
PDB entry 4i7h coloured by chain, top view.
Source organism: Streptococcus pyogenes MGAS315
Primary publication:
Crystal structure of peroxide stress regulator from Streptococcus pyogenes provides functional insights into the mechanism of oxidative stress sensing.
Makthal N, Rastegari S, Sanson M, Ma Z, Olsen RJ, Helmann JD, Musser JM, Kumaraswami M
J Biol Chem 288 18311-24 (2013)
PMID: 23645680

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ferric uptake regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.9 KDa
Source organism: Streptococcus pyogenes MGAS315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UT39 (Residues: 1-155; Coverage: 100%)
Gene names: SpyM3_0147, spf
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6322
Unit cell:
a: 118.91Å b: 118.91Å c: 118.611Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.229 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Hydrogen peroxide sensing, signaling and regulation of transcription factors.
Marinho et al. (2014)
6 more

6 mentions without citation

How Bacterial Redox Sensors Transmit Redox Signals via Structural Changes.
Lee et al. (2021)
5 more