› 4i8b

X-ray diffraction
2Å resolution

Crystal Structure of Thioredoxin from Schistosoma Japonicum

Released:
DOI: 10.2210/pdb4i8b/pdb
PDB entry 4i8b coloured by chain, front view.
PDB entry 4i8b coloured by chain, side view.
PDB entry 4i8b coloured by chain, top view.
Source organism: Schistosoma japonicum
Primary publication:
Expression, characterization and crystal structure of thioredoxin from Schistosoma japonicum.
Li Y, Li P, Peng Y, Wu Q, Huang F, Liu X, Li X, Zhou H, Guo D, Shi D, Zhou XN, Fan X
Parasitology 142 1044-52 (2015)
PMID: 25810021

Function and Biology Details

Reaction catalysed: 
Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 11.74 KDa
Source organism: Schistosoma japonicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U8F3 (Residues: 2-106; Coverage: 99%)
Gene name: EWB00_003879
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 90.756Å b: 149.364Å c: 39.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The Architecture of Thiol Antioxidant Systems among Invertebrate Parasites.
Guevara-Flores et al. (2017)
4 other articles