› 4i8t

X-ray diffraction
3Å resolution

C.Esp1396I bound to a 19 base pair DNA duplex

Released:
DOI: 10.2210/pdb4i8t/pdb
PDB entry 4i8t coloured by chain, front view.
PDB entry 4i8t coloured by chain, side view.
PDB entry 4i8t coloured by chain, top view.
Source organism: Enterobacter sp. RFL1396
Primary publication:
Structural analysis of DNA-protein complexes regulating the restriction-modification system Esp1396I.
Martin RN, McGeehan JE, Ball NJ, Streeter SD, Thresh SJ, Kneale GG
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 962-6 (2013)
PMID: 23989141

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117634 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.52 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(P*TP*GP*TP*TP*GP*AP*CP*TP*AP*TP*AP*AP*TP*CP*AP*CP*AP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.79 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided
DNA (5'-D(P*TP*GP*TP*GP*TP*GP*AP*TP*TP*AP*TP*AP*GP*TP*CP*AP*AP*CP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.86 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 75.51Å b: 60.86Å c: 80.35Å
α: 90° β: 113.47° γ: 90°
R-values:
R R work R free
0.241 0.238 0.293
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 citation in other articles

Transcriptome analyses of cells carrying the Type II Csp231I restriction-modification system reveal cross-talk between two unrelated transcription factors: C protein and the Rac prophage repressor.
Negri et al. (2019)
2 more

1 mention without citation

Structural analysis of DNA-protein complexes regulating the restriction-modification system Esp1396I.
Martin et al. (2013)